BIOACTIVITY OF ESSENTIAL OILS FROM PIPERACEAE ON THE DEVELOPMENT OF SPODOPTERA FRUGIPERDA (LEPIDOPTERA: NOCTUIDAE) UNDER LABORATORY CONDITIONS.
Ovicidal activity; Pest management; Larval toxicity.
The fall armyworm, Spodoptera frugiperda, is a pest of major economic importance in agricultural crops, requiring sustainable alternatives to conventional chemical control. This study evaluated the bioactivity of essential oils (EOs) from seven Piper species on the development of S. frugiperda under laboratory conditions. Ovicidal bioassays were conducted using 0–24-hour-old eggs fixed on cardboard and sprayed with EO concentrations ranging from 0.25% to 8%, and larvicidal bioassays were performed by topically applying 2 µL of the dilutions onto second-instar larvae. A completely randomized design was used, with ten replicates for the ovicidal bioassay and twenty for the larvicidal assay, including negative controls with distilled water and acetone. Chemical characterization of the EOs was carried out by gas chromatography coupled with mass spectrometry. Data were subjected to ANOVA and LSD test, and lethal concentrations were calculated by probit analysis. The results demonstrated a dose-dependent effect for both activities. In the ovicidal activity, P. marginatum showed inviability ranging from 50% to nearly 100% at concentrations of 1% to 8%, whereas P. divaricatum exhibited significant effects only at 8%. Chemical analysis revealed the predominance of sesquiterpenes such as Germacrene B and D in some species, and volatile monoterpenes such as α-pinene in others. In the larvicidal activity, P. marginatum, P. divaricatum, and P. fuligineum reached 100% mortality at 8% within 24 hours. It is concluded that Piper essential oils, particularly those rich in sesquiterpenes, show potential for the development of biopesticides.