Banca de QUALIFICAÇÃO: ADRIELY LUIZA FERREIRA

Uma banca de QUALIFICAÇÃO de MESTRADO foi cadastrada pelo programa.
DISCENTE : ADRIELY LUIZA FERREIRA
DATA : 10/10/2025
HORA: 09:00
LOCAL: Sala de Aula CELBE
TÍTULO:

Histophysiological aspects at different stages of gonadal development of Pseudoplatystoma corruscans in its natural habitat in the Northern Pantanal, Mato Grosso, Brazil.

PALAVRAS-CHAVES:

Immunoassay, hormones, pintado.

PÁGINAS: 74
GRANDE ÁREA: Ciências Biológicas
ÁREA: Zoologia
SUBÁREA: Fisiologia dos Grupos Recentes
RESUMO:

Pseudoplatystoma corruscans is a species of economic interest in the state of Mato Grosso and is therefore included in reproductive and population monitoring programs of wild species. In this activity, challenges arise in sex identification and reproductive staging, since the species does not present external sexual dimorphism. There are records of sex determination through technological methods such as ultrasonography, endoscopy, and histological analysis by abdominal incision. However, these techniques are invasive and/or present limitations regarding their effectiveness under field conditions. In this context, the present work was structured and developed in the form of two integrative articles. The first article, a narrative review, sought to evaluate innovative non-lethal techniques for sexing and reproductive staging of fish, while the second aimed to validate an immunobiochemical technique as a non-invasive tool for sexing wild P. corruscans. In the first article, through a literature survey, the main techniques used were identified, highlighting endoscopy and ultrasonography, which enabled the visualization of reproductive organs without causing death in specimens, but proved to be highly invasive and not very accurate, respectively. It was also observed that molecular and immunoassay methods are reliable for sex determination and reproductive staging, as they are less invasive and practical in field applications. The second article aimed to validate the enzyme-linked immunosorbent assay (ELISA) with plasma levels of 17β-estradiol (E2), testosterone (T), and 11-ketotestosterone (11-KT) in wild male and female P. corruscans. Hormonal analyses revealed marked differences between sexes and gonadal development stages in P. corruscans. In males, T and 11-KT concentrations were significantly higher during maturation, confirming 11-KT as the main androgen associated with spermatogenesis and reproductive peak. In females, discrete increases in testosterone were observed during maturation, while 11-KT remained at low levels in both stages. These patterns reinforce sexual distinction in the androgenic balance, with 11-KT acting as the main marker of male reproductive activity and testosterone playing a more modest, yet relevant, role in female gonadal development.

MEMBROS DA BANCA:
Presidente - 65536013 - CLAUMIR CESAR MUNIZ
Interno - 118188003 - ERNANDES SOBREIRA OLIVEIRA JUNIOR
Externa à Instituição - GIOVANA SOUZA BRANCO - USP
Interno - 118181001 - WILKINSON LOPES LAZARO