PLASMA STEROIDS AS NON-LETHAL MARKERS OF SEX AND REPRODUCTIVE CONDITION OF Piaractus mesopotamicus (HOLMBERG, 1887)
Estradiol; testosterone; 11-ketotestosterone; staging; Pacu; Pantanal.
The pacu, Piaractus mesopotamicus, a species of commercial importance for fishing and fish farming, does not present evident external sexual dimorphism, and the identification of sex and reproductive stage generally depends on lethal methods. This study evaluated whether plasma concentrations of 17β-estradiol (E2), testosterone (T) and 11-ketotestosterone (11KT), and the ratios between them, distinguish the sex and reproductive condition of the species in a natural environment, comparing their performance to lethal indices. 61 specimens (31 females and 30 males) were sampled at the Taiamã Ecological Station, Pantanal of Mato Grosso. Sex and gonadal stage (resting, maturing and emptied) were determined by macroscopic inspection, a subsample of the gonads was characterized by histology and hormones were quantified by ELISA. Maturing and emptied animals were subsequently grouped as active and compared to inactive (resting). 11KT was the best marker to distinguish the sexes, with a median around 5 times higher in males (p < 0.001) and almost complete separation. In the 19 females with valid dosage, E2 completely separated the active from the inactive (p < 0.001), while the gonadosomatic index showed overlap between the groups. In males, T and 11KT were approximately 3.5 and 22 times higher in active animals (p < 0.001), and the 11KT/T ratio separated the groups almost completely, although the active ones were almost all depleted. Plasma steroids are promising candidates for non-lethal markers of pacu sex and reproductive status, but the observed cutoffs must be validated in independent samples.