TEAK CANCER: CULTURAL CHARACTERIZATION OF Lasiodiplodia theobrome AND PATHOGENICITY TEST
Tectona grandis; Lasiodiplodia theobrome; morphological characterization; pathogenicity
In Brazil, the presence of L. theobromae causing cancer and even death in Tectona grandis was identified, in this
sense the objective of this study is to carry out the characterization of Lasiodiplodia theobromae associated with
the damage caused in Tectona grandis and to identify the potential severity of the isolates collected in the state
from Mato Grosso and Pará. The study will be divided into two stages, the first being conducted at the Laboratory
of Plant Genetic Improvement with the morphological characterization of samples collected from symptomatic teak
plants and isolated in culture medium with subsequent identification under an optical microscope, for cultural
characterization of the isolates, will be An experiment was set up in a 6 x 3 x 2 factorial arrangement
(temperature, culture medium, photoperiod) and every 24 hours the mycelial growth diameter (DM) will be
measured, until the colony completely covers the diameter of the Petri dish, quantifying the number of conidia
with the aid of a Neubauer chamber. In this way the average mycelial growth rate will be determined.
The analysis of variance and the means when significant will be compared by the Scott-Knott test with a
probability of 5% using the Sisvar computer program. The second stage will be carried out in a greenhouse
with an aggressiveness and pathogenicity test of the different isolates inoculated in UNEMAT 33 genotype.
The plants will be evaluated at 150 days after the fungus inoculation, the internal lesion length (CL) will be
measured by longitudinal section. of the stem of the seedlings and evaluation of the lesion area (AL). The data
obtained will be applied by the Scott-Knott test at the level of 5% of probability for the comparison of the
means using the static program Sisvar.