Banca de DEFESA: EDNAN HENRIQUE ALVES DE OLIVEIRA

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
DISCENTE : EDNAN HENRIQUE ALVES DE OLIVEIRA
DATA : 18/08/2025
HORA: 08:00
LOCAL: Sala da Pós Graduação - PGMP - CIP Cerrado
TÍTULO:

Germination and in vitro regeneration of Brazilnuttree(Bertholletia excelsa H.B.K)

PALAVRAS-CHAVES:

Germination; Organogenesis Rhizogenesis; Microporous Membranes

PÁGINAS: 54
GRANDE ÁREA: Ciências Agrárias
ÁREA: Agronomia
SUBÁREA: Fitotecnia
ESPECIALIDADE: Melhoramento Vegetal
RESUMO:

The species Bertholletia excelsa, known as the Amazon nut, plays an essential role in the economy and environmental conservation of the Amazon region. However, its propagation is limited by the low germination rate and slow growth of seedlings. The present study investigates the in vitro germination of seeds of the genotypes of B. excelsa (Caracaraí VIC-ITÂ P-26, Caracaraí VIC-ITÂ P-65, Caracaraí VIC-ITÂ P-71) in multistem development and regeneration from mini-cuttings of the genotype Caracaraí VIC-ITÂ P-71 and rooting with the use of microporous membranes. In seed germination, the genotype Caracaraí VIC-ITÂ P-26 obtained a percentage of 13.3% of germinated seeds, being 16.6% and 36.6% for the respective genotypes Caracaraí VIC-ITÂ P-65, Caracaraí VIC-ITÂ P-71. For the development of multistems, the Caracaraí VIC-ITÂ P-71 genotype obtained a higher yield: 20%. In the in vitro regeneration of  mini-cuttings, the presence of BA and TDZ promoted the formation of newly formed shoots. At concentrations of 1.0 mgL^-1 TDZ produced the highest percentage of shoots with 73.3%, followed by BA at 0.25 mgL^-1 with 40%, after 60 days of in vitro culture. In the rooting process, the combination of 0.50 mgL⁻¹ of IBA with caps containing only one microporous membrane (1M) presented a percentage of 83.3% of rooted mini-cuttings, and with 0.50 mgL^-1 2M IBA and 1.0 mgL-1 1M IBA obtained a percentage of 16.6%, respectively. Regarding the length of the roots, the highest average was 7.63 mm in control treatment with caps without membrane. The best response for leaf production occurred in control treatment (SM) with a percentage of 100%. In terms of leaf length and diameter, the best mean was obtained with 1.0 mgL⁻¹ of IBA and caps with 2M showing 18.13 mm and 5.85 mm, respectively. For the cultivation of mini-cuttings a of B. It is also suggested the use of 1.0 mgL-1 TDZ to induce the regeneration of neoformed buds and for the development of a complete plant, the addition of 0.50 mgL⁻¹ of IBA and the use of caps in culture flasks with only one microporous membrane. In vitro regeneration and obtaining complete plants contribute significantly to expanding the methods of propagation B. excelsa.

MEMBROS DA BANCA:
Interna - 59687004 - MAURECILNE LEMES DA SILVA CARVALHO
Externo ao Programa - 131840001 - ILIO FEALHO DE CARVALHO
Externa à Instituição - CASSIA ÂNGELA PEDROZO - EMBRAPA
Externo à Instituição - 067.531.536-08 - DIEGO ISMAEL ROCHA - UFV